Pay Grade: 39
Hiring Range: $19.27/Hourly
JOB SUMMARY/ESSENTIAL JOB FUNCTIONS: Under the direction of an assigned supervisor, this position works within the Microbiology, Immunology, and Biochemistry Department (MIB) and is responsible for general laboratory oversight, conducting experiments using standard immunological techniques, analyzing, recording, and preparing reports/graphs of experimental data from experiments using human or mouse tissues or mouse models of inflammation or infection. Supervises medical students and undergraduate students doing summer/year-round research projects in the laboratory.
DUTIES AND RESPONSIBILITIES:
- Generates Tumor Infiltrating T-cell lines from patients with various types of tumors; characterizes the cell lines using flow cytometry and intracellular cytokine staining; and generates single cell T-cell clones.
- Prepares RNA or DNA for sequencing or performs qRT-PCR to measure the level of various inflammatory mediators or checkpoint inhibitor proteins and their receptors.
- Works with mouse models of inflammation to identify the genetic basis of response to anti-inflammatory therapeutics.
- Performs ELISA or bead-based arrays on tissues from human or mouse origin to measure inflammatory response.
- Designs or optimizes assays; develops protocols or standard operating procedures for all techniques used; uses statistical analysis tools to analyze data; and generates graphs and reports from data.
- Performs general laboratory housekeeping tasks orders supplies and keeps inventory train medical or graduate students in basic techniques.
- Manages equipment service inspections, training of individuals, and maintenance of equipment.
- Maintains inventory of supplies, reagents, cell lines, freezer contents, etc.
- Performs other related duties as assigned.
EDUCATION: Bachelor's Degree in one of the sciences. (TRANSCRIPT REQUIRED)
EXPERIENCE: Three (3) years of prior research laboratory experience; OR Master's Degree and one (1) year of strong experience with BSL2 level sterile cell culture techniques. (Experience with standard immunologic techniques preferred). (Three (3) or more years of related laboratory experience beyond a Bachelor's Degree preferred).KNOWLEDGE, SKILLS, AND ABILITIES:
- Ability to communicate effectively, both orally and in writing.
- Possess strong organizational and time management skills.
- Ability to work independently and strong interpersonal skills to work effectively as part of a team.
- Basic knowledge of flow cytometry.
- Ability to learn graphical and statistical computer software available in lab.
- Possess good analytical skills.
WORK SCHEDULE: This position may be occasionally required to work weekends.
An official transcript or documentation to verify education must be submitted in a sealed envelope to the Human Resources Department prior to first day of employment if selected for a position.
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